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. 2012 Mar 19;287(19):15672–15683. doi: 10.1074/jbc.M111.322669

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Up-regulation of miR-132 and miR-212 by Ang II in RVSMC. A, levels of miR-132 and miR-212 were analyzed by RT-qPCR in Ctrl or Ang II-treated (100 nm) RVSMC at the indicated time points, and results are expressed as % of Ctrl (**, p < 0.01; ***, p < 0.001, n = 8). B, expression of miR-132/212 in RVSMC treated with Ang II at the indicated concentrations for 6 h was determined by RT-qPCR. Results are shown as % of Ctrl (**, p < 0.01; ***, p < 0.001, n = 6). C, levels of miR-132 and miR-212 in RVSMC pretreated with vehicle or Losartan (10 μm) for 30 min and then treated with Ang II (100 nm) at indicated time points were determined by RT-qPCR. Data are from one experiment performed in duplicate.