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. 2012 Mar 19;287(19):15672–15683. doi: 10.1074/jbc.M111.322669

FIGURE 3.

FIGURE 3.

miR-132 directly targets PTEN in RVSMC. A, shown is sequence alignment of four potential miR-132 binding sites in rat PTEN 3′-UTR predicted by TargetScan. Nucleotide locations of the four binding sites are shown. B, PTEN mRNA levels were determined by RT-qPCR in Ctrl and Ang II-treated RVSMC at the indicated time points. Results were expressed as % of Ctrl (**, p < 0.01; ***, p < 0.001, n = 6). C, PTEN mRNA levels in RVSMC transfected with NC-M or 132-M. Data were expressed as % of NC-M (**, p < 0.01, n = 6). D, shown are representative immunoblots of PTEN protein in RVSMC transfected with NC-M or 132-M in duplicate samples. β-Actin was used as the internal control. The bar graph (right panel) shows quantification of PTEN protein levels (**, p < 0.01, n = 6). E and F, shown is relative activity of luciferase (luc) reporter with PTEN 3′-UTR containing miR-132 binding sites co-transfected with the indicated mimic (E) or inhibitor (F) oligonucleotides in RVSMC. Results were shown as % of respective NC oligonucleotides (***, p < 0.001, n = 8).