Table 4.
Donor | Recipient | Selection | Reversion frequency of selected marker | #Colonies | Frequency, cfu/cfu | Frequency, cfu/pfu |
---|---|---|---|---|---|---|
DAH2 | ||||||
J222 | 2709 | Arg+ | <1 × 10−7 | 11 | 1 × 10−6 | 3 × 10−7 |
J222 | 2709 | Pro+ | <1 × 10−7 | 10 | 1 × 10−6 | 3 × 10−6 |
J222 | 2709 | His+ | <1 × 10−7 | 31 | 3 × 10−6 | 8 × 10−7 |
J222 | A3(2) | Rifr | 1 × 10−8 | 34 | 3 × 10−7 | 5 × 10−7 |
1258 | J222 | Strr | 3 × 10−8 | 0 | 0 | 0 |
YU105 | BldK∷Ω | Lincr | 1 × 10−8 | 0 | 0 | 0 |
YU105 | J222 | Hygr | 1 × 10−6 | 401 | 4 × 10−5 | 3 × 10−5 |
YU105 | J2402 | Eryr | 1 × 10−7 | 62 | 6 × 10−6 | 7 × 10−9 |
DAH4 | ||||||
J222 | 2709 | Arg+ | <1 × 10−8 | 310 | 3 × 10−6 | 5 × 10−6 |
J222 | 2709 | His+ | <1 × 10−8 | 210 | 2 × 10−6 | 5 × 10−5 |
J222 | 2709 | Pro+ | <1 × 10−8 | 120 | 1 × 10−6 | 3 × 10−6 |
J222 | A3(2) | Rifr | 1 × 10−8 | 123 | 1 × 10−6 | 3 × 10−6 |
1258 | J222 | Strr | 3 × 10−8 | 409 | 4 × 10−6 | 2 × 10−6 |
YU105 | J2402 | Eryr | 1 × 10−8 | 311 | 3 × 10−6 | 2 × 10−7 |
DAH5 | ||||||
J222 | 2709 | Arg+ | <1 × 10−8 | 12 | 1 × 10−7 | 3 × 10−6 |
J222 | 2709 | His+ | <1 × 10−8 | 10 | 1 × 10−7 | 3 × 10−7 |
J222 | 2709 | Pro+ | <1 × 10−8 | 21 | 2 × 10−7 | 7 × 10−6 |
J222 | A3(2) | Rifr | 1 × 10−8 | 30 | 3 × 10−7 | 7 × 10−6 |
1258 | J222 | Strr | 3 × 10−8 | 31 | 3 × 10−6 | 2 × 10−6 |
YU105 | BldK∷Ω | Lincr | 1 × 10−8 | 30 | 3 × 10−7 | 2 × 10−8 |
YU105 | J222 | Hygr | 1 × 10−6 | 62 | 6 × 10−6 | 5 × 10−6 |
YU105 | J2402 | Eryr | 1 × 10−8 | 93 | 9 × 10−7 | 2 × 10−7 |
DAH6 | ||||||
J222 | 2709 | Arg+ | <1 × 10−8 | 21 | 2 × 10−7 | 5 × 10−6 |
J222 | 2709 | His+ | <1 × 10−8 | 30 | 3 × 10−7 | 7 × 10−6 |
J222 | 2709 | Pro+ | <1 × 10−8 | 23 | 2 × 10−7 | 5 × 10−6 |
J222 | A3(2) | Rifr | 1 × 10−8 | 123 | 1 × 10−6 | 3 × 10−6 |
1258 | J222 | Strr | 3 × 10−8 | 33 | 3 × 10−7 | 7 × 10−6 |
YU105 | BldK∷Ω | Lincr | 1 × 10−8 | 30 | 3 × 10−7 | 3 × 10−8 |
YU105 | J222 | Hygr | 1 × 10−6 | 403 | 4 × 10−5 | 7 × 10−5 |
YU105 | J2402 | Eryr | 1 × 10−8 | 42 | 4 × 10−7 | 3 × 10−9 |
Rifampicin 150 μg/ml; streptomycin 25 μg/ml; erythromycin 100 μg/ml; hygromycin 25 μg/ml; lincomycin 150 μg/ml.