Table 5.

Cotransduction by DAH4, DAH5, and DAH6

Phage	Donor	Recipient	Selection	Spontaneous resistance to selected marker	#Colonies	Transduction frequency, cfu/cfu	Unselected marker	#Colonies	Cotransduction frequency, %
DAH4*	1258	J222	Strr	<1  × 10−8	335	7.4  × 10−7	Rifs	225	67
DAH4*	J222	1258	Rifr	2.9  × 10−8	67	9.3  × 10−7	Strs	39	59
DAH6*	1258	J222	Strr	<1  × 10−8	370	9.9  × 10−6	Rifs	232	63
DAH6*	J222	1258	Rifr	2.9  × 10−8	78	6.7  × 10−7	Strs	51	66
DAH4†	YU105	BldK∷Ω	Lincr	1.1  × 10−8	163	3  × 10−7	Spcs	87	53
DAH5†	YU105	BldK∷Ω	Lincr	1.1  × 10−8	213	3  × 10−7	Spcs	125	59
DAH6†	YU105	BldK∷Ω	Lincr	1.1  × 10−8	142	3  × 10−8	Spcs	56	39

^*Streptomycin selected at 25 μg/ml; rifampicin test for sensitivity at 50 μg/ml; rifampicin selected at 40 μg/ml; streptomycin test for sensitivity at 40 μg/ml. 

^† Lincomycin selection at 150 μg/ml; test for spectinomycin sensitivity at 50 μg/ml.