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. 2012 Mar 12;287(19):15851–15861. doi: 10.1074/jbc.M111.281832

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Analysis of the Triton X-100 solubility of SUMOylated Cx43. HeLa cells were transfected with Cx43; HA-Ubc9; and either HA-SUMO-1, -2, or -3. Cells were then subjected to a Triton X-100 solubility assay. The total cell lysate fractions (T), the Triton X-100-soluble fractions (S) and the Triton X-100-insoluble fractions (I) were subjected to immunoprecipitation using anti-Cx43 antibodies. Immunoprecipitates were subjected to SDS-PAGE and SUMOylated Cx43 was detected using anti-HA antibodies. The blots were stripped and reprobed with anti-Cx43 antibodies. Molecular mass in kDa is indicated.