TABLE 2.

Comparison of qRT-PCR standard curves of ECS elements in human NHEM cells

Gene	Slope	r2	Ct	Copy numbersa	mRNA copiesb
CB1	−1.546	0.996	32.83 ± 0.27	13 ± 0.11	3.58e−06
CB2	−1.196	0.973	28.41 ± 0.03	16 ± 0.02	4.36e−06
TRPV1	−1.546	0.996	21.99 ± 0.16	14280 ± 103.9	3.98e−03
NAPE-PLD	−1.412	0.976	20.28 ± 0.04	5817 ± 11.47	1.62e−03
FAAH	−1.374	0.999	23.27 ± 0.23	1122 ± 11.09	3.13e−04
MAGL	−1.444	0.960	19.04 ± 0.03	11648 ± 18.35	3.24e−03
DAGL	−1.651	0.976	26.98 ± 0.19	456 ± 3.21	1.27e−04

^a Values are per 50 ng of cDNA.

^b Data were normalized to gene copies of the housekeeping gene β-actin.