TABLE 4.

Levels of pCREB in human NHEM cells treated with mAEA, α-MSH, and related compounds

SR141716, SB203580, PD98059, and H89 were ineffective when used alone under the same experimental conditions (omitted for the sake of clarity).

NHEM treatment	pCREB level (A450 nm)
Ctrl	0.347 ± 0.034
mAEA (1 μm)	0.749 ± 0.055a
mAEA (1 μm) + SR141716 (0.1 μm)	0.415 ± 0.092b
mAEA (1 μm) + SB203580 (10 μm)	0.476 ± 0.063c
mAEA (1 μm) + PD98059 (10 μm)	0.409 ± 0.015b
mAEA (1 μm) + H89 (5 μm)	0.691 ± 0.079a
α-MSH (100 nm)	0.702 ± 0.111a
α-MSH (100 nm) + H89 (5 μm)	0.383 ± 0.035d

^a p < 0.01 versus control (Ctrl).

^b p < 0.01 versus mAEA.

^c p < 0.05 versus mAEA.

^d p < 0.05 versus α-MSH.