FIGURE 3.

Surface and intracellular pools of NEU1 and NEU3. A, nonpermeabilized HMVEC-Ls were studied by flow cytometry for surface NEU1 and NEU3. B, permeabilized HMVEC-Ls were studied by flow cytometry for total NEU1 and NEU3. HMVEC-Ls, some of which were permeabilized with Triton X-100, were incubated with rabbit polyclonal antibodies raised against human NEU1 or NEU3 or a species-matched control antibody, washed, and incubated with FITC-conjugated goat anti-rabbit antibody. Each plot is representative of three or more independent experiments.