FIGURE 1.

Circulatory Fc sialylation in NTHi-mediated pulmonary inflammation. C57BL/6 mice (WT) were subjected to biweekly intra tracked instillations of NTHi for 16 consecutive weeks as detailed under “Experimental Procedures,” and serum (n = 5) was harvested weekly and pooled (where D1 represents a post-challenge blood draw on the first day of treatment). Fc fragments of IgG were isolated from each of the weekly pools, subjected to SDS-PAGE, and probed with SNA (green), anti-Fc antibody (blue), and anti-Fab antibody (red) as displayed in the inset. The SNA reactivity of the Fc fragments is expressed as a ratio of the relative SNA to anti-Fc signals, where untreated denotes the ratio found in the serum IgG of animals without treatment.