FIGURE 2.

Circulatory Fc sialylation in OVA model of allergic pulmonary inflammation. C57BL/6 (WT) and Siat1ΔP1 mice were sensitized to OVA and subjected to acute and chronic protocols of pulmonary inflammation as described under “Experimental Procedures.” Sera from five animals representing each of the conditions were pooled, and Fc fragments of IgG were isolated from pooled sera from animals sensitized but not challenged by OVA (Primed), challenged with the acute protocol (Acute), and challenged with the chronic protocol (Chron). A, sera were subjected to SDS-PAGE analysis, probed with SNA or ECL (green), anti-Fc antibody (blue), and anti-Fab antibody (red). Resol, resolution. B, quantitation of the data from A expressed as SNA/anti-Fc or ECL/anti-Fc ratios. Ratio values <30 were regarded as random noise.