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PSL and ECL reactivity of Fc fragments. Fc fragments from serum pools of five C57BL/6 (WT), Siat1ΔP1 (ΔP1), and Siat1-null (KO) mice were subjected to SDS-PAGE and blotted for reactivity against PSL (A) and ECL (B), recognizing the α2,6-sialyl type 2 lactosamine and unsialylated type 2 lactosamine structures, respectively, as diagrammed.
