TABLE 2.
Specific activity values for P4-P3′ factor VIII variants
Specific activity values were determined through one state clotting assay as described under “Experimental Procedures” and represent mean ± S.D. from at least three separate determinations. Factor VIIIa values were obtained from a ∼1–2-min time point following addition of thrombin.
| Factor VIII | Specific activity | Activity following thrombin activation |
|---|---|---|
| units/μg | ||
| WT | 4.5 ± 0.4 (1.0)a | 120.0 ± 12.7 (1.0) |
| 336(P4-P2)372 | 2.6 ± 0.3 (0.6) | 53.4 ± 4.4 (0.5) |
| 336(P1′-P3′)372 | 4.5 ± 0.5 (1.0) | 75.4 ± 9.8 (0.6) |
| 336(P4-P3′)372 | 3.5 ± 0.2 (0.8) | 64.2 ± 4.2 (0.5) |
| 336(P4-P2)740 | 5.8 ± 0.7 (1.3) | 200.2 ± 31.8 (1.7) |
| 336(P1′-P3′)740 | 4.6 ± 0.4 (1.0) | 75.1 ± 6.4 (0.6) |
| 336(P4-P3′)740 | 2.4 ± 0.2 (0.5) | 57.5 ± 11.2 (0.5) |
| 336(P4-P3′)562 | 5.2 ± 0.3 (1.2) | 142.1 ± 20.6 (1.2) |
| R336Q | 3.8 ± 0.2 (0.8) | 89.9 ± 5.3 (0.8) |
a Values in parentheses are activity values relative to wild type.