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Phenotypic characterization of hcm knock-out mutants. Nearly stoichiometric conversion of TBA (A) and 2-methylpropan-1,2-diol (B) to 2-HIBA by resting cells of the knock-out mutant strains L108(ΔhcmB)K7 and L108(ΔhcmA)K5 pre-grown on tert-amyl alcohol (mean values of experiments performed with both mutants) is shown. In the case of incubation with TBA, also small amounts of 2-methylpropan-1,2-diol accumulated.
