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Potential membrane topologies of members of the GAAP family. A, amino acid sequence of VACV strain Evans GAAP and TMDs predicted by TMHMM 2.0 (blue), MEMSAT-SVM (red), TopPred 0.01(green) and SOSUI (orange). B, schematic illustration of hGAAP and BI-1 transmembrane regions (TM1–TM7) predicted by MEMSAT-SMV (black) and an additional transmembrane region predicted by MEMSAT3 (gray). Positions of individual HA tags insertions into VACV GAAP and BI-1 are indicated (black triangles).
