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. 2012 Mar 8;287(19):15174–15192. doi: 10.1074/jbc.M111.308023

FIGURE 1.

FIGURE 1.

Expression of RRM1 and RRM2 in mouse uterus during early pregnancy. A, both mRNA and protein localizations of RRM1 and RRM2 in mouse uterus from day 5 (D5) to day 8 (D8) were detected by in situ hybridization and immunostaining, respectively. Real-time RT-PCR was performed to quantify the mRNA level of Rrm1 (B) and Rrm2 (C) in mouse uterus on day 5 (D5NI, interimplantation site on day 5; D5I, implantation site on day 5) and day 8. D, Northern blot. Both long transcripts (LT) and small transcripts (ST) of Rrm2 were up-regulated at the implantation sites. 28S was used as internal reference. E, RT-PCR analysis of Rrm2 mRNA level in mouse pregnant uteri. Strong Rrm2 bands were observed in implantation sites on days 5 and 8 compared with the interimplantation site on day 5 of pregnancy. F, Western blot of RRM1 and RRM2 protein in mouse uterus on days 5 and 8. G, physical interaction between RRM1 and RRM2 in mouse decidua was detected by co-immunoprecipitation. ge, glandular epithelium; le, luminal epithelium; my, myometrium; st, stroma; pd, primary decidua; sd, secondary decidua; arrow, embryo. Bar, 300 μm. *, p < 0.05; error bars, S.E.