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. 2012 Mar 8;287(19):15174–15192. doi: 10.1074/jbc.M111.308023

FIGURE 11.

FIGURE 11.

Expression of CHK1, E2F1, and RRM2 in stromal cells treated with CPT or UV. A, real-time RT-PCR of Chk1, E2f1, and Rrm2 mRNA levels after CPT (1 μm)-treated stromal cells were pretreated with caffeine (inhibitor of ATR/ATM), KU-55933 (inhibitor of ATM), or UCN-01 (inhibitor of CHK1), respectively. B, real-time RT-PCR of Chk1, E2f1, and Rrm2 mRNA levels after UV (20 J)-treated stromal cells were pretreated with caffeine, KU-55933, or UCN-01, respectively. C, Western blot of RRM2 and p-CHK1 proteins after CPT-treated stromal cells were pretreated with caffeine, KU-55933, or UCN-01, respectively. D, Western blot of RRM2 and p-CHK1 proteins after UV-treated stromal cells were pretreated with caffeine, KU-55933, or UCN-01, respectively. *, p < 0.05; error bars, S.E.