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. 2012 Mar 8;287(19):15174–15192. doi: 10.1074/jbc.M111.308023

FIGURE 12.

FIGURE 12.

E2F1 regulation on RRM2 expression. A, real-time RT-PCR of E2f1 mRNA level in E2f1-overexpressing stromal cells. B, the relative luciferase activity of Rrm2 promoter in E2f1-overexpressing stromal cells. C, real-time RT-PCR of Rrm2 mRNA level in E2f1-overexpressing stromal cells. D, Western blot of RRM2 protein in E2f1-overexpressing stromal cells. E, real-time RT-PCR of E2f1 mRNA expression after stromal cells were transfected with E2f1 siRNA. F, Western blot of RRM2 protein after stromal cells were transfected with E2f1 siRNA (siE2f1) and then treated with CPT. *, p < 0.05; error bars, S.E.