FIGURE 13.

CHK1 regulation on RRM2 expression. A, Western blot of p-CHK1 protein after ovariectomized mice were treated with progesterone or progesterone plus RU486. B, Western blot of RRM2 protein after progesterone-treated stromal cells were pretreated with UCN-01, RU486, or UCN-01 plus RU486. C, real-time RT-PCR of Rrm2 mRNA expression after stromal cells were treated with progesterone (P4), UCN-01, 10058-F4, or a combination of them. D, Western blot analysis of RRM2 protein expression after stromal cells were treated with progesterone (P4), UCN-01, 10058-F4, or a combination of them. *, p < 0.05; error bars, S.E.