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. 2012 Mar 8;287(19):15174–15192. doi: 10.1074/jbc.M111.308023

FIGURE 2.

FIGURE 2.

Both mRNA and protein expressions of RRM1 and RRM2 at implantation sites in mouse uteri under delayed implantation and artificial decidualization, respectively. A, in situ hybridization and immunostaining of RRM1 and RRM2 under delayed implantation and artificial decidualization, respectively. B, real-time PCR analysis of Rrm1 under artificial decidualization. C, real-time PCR analysis of Rrm2 mRNA expression under artificial decidualization. D, RT-PCR analysis of Rrm2 mRNA level. Rrm2 mRNA level in mouse deciduoma was significantly higher compared with control. Gapdh was used for loading control. E, Western blot analysis of RRM2 protein. RRM2 protein level in deciduoma was significantly stronger than control. ge, glandular epithelium; le, luminal epithelium; my, myometrium; st, stroma; pd, primary decidua; sd, secondary decidua; arrow, embryo. Bar, 300 μm. *, p < 0.05; error bars, S.E.