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. 2012 Mar 8;287(19):15174–15192. doi: 10.1074/jbc.M111.308023

FIGURE 4.

FIGURE 4.

Effects of hydroxyurea on cell proliferation and decidualization in vitro. A, MTT assay of cell proliferation after stromal cells were treated with different concentrations of hydroxyurea. B, flow cytometry analysis of cell cycle after stromal cells were treated with hydroxyurea. C, histogram of the stromal cells under different cell cycles after stromal cells were treated with hydroxyurea. D, real-time RT-PCR of Rrm1 mRNA expression under in vitro decidualization. E, real-time RT-PCR of Rrm2 mRNA expression under in vitro decidualization. F, real-time RT-PCR of Dtprp mRNA expression under in vitro decidualization. Con, control; E2 + P4, estradiol-17β plus progesterone; E2 + P4 + HU, estradiol-17β plus progesterone plus hydroxyurea. *, p < 0.05; error bars, S.E.