FIGURE 9.

The involvement of p-AKT and c-MYC in progesterone-induced RRM2 expression. A, the relative luciferase activity of Rrm2 promoter after c-Myc was overexpressed in stromal cells. B, Western blot of c-MYC and RRM2 in progesterone-treated stromal cells after pretreatment with 20 or 40 μm 10058-F4, an inhibitor of c-Myc. 10058-F4 can block the role of progesterone on c-Myc and RRM2. C, ChIP analysis. Uterine decidua from implantation sites on day 8 were used for chromatin immunoprecipitation with anti-c-Myc or anti-acetylated H3 (AcH3) antibody (a positive control for active transcription). IgG was used for a negative control. D, effects of RU486 and Ly294002 on progesterone-induced RRM2 expression. Stromal cells were pretreated with RU486 or Ly294002 for 1 h and then treated with progesterone. *, p < 0.05; error bars, S.E.