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. 2012 Mar 13;287(19):15776–15785. doi: 10.1074/jbc.M112.341594

FIGURE 7.

FIGURE 7.

Functions of different regions of PfN23. A, schematic representation of truncations and peptides. Numbers indicate the position of amino acid. B and C, accelerative activities of truncations and peptides during calcium carbonate precipitation. Changes in the turbidity of the assayed solutions are shown. B, activities of peptides. ■, 10 μg/ml Pep-N; ●, 10 μg/ml Pep-M; ▴, 10 μg/ml Pep-C was present; ▾, 10 μg/ml BSA was used as a negative control. C, activities of truncations. ■, 10 μg/ml ΔN; ●, 10 μg/ml ΔM; ▴, 10 μg/ml ΔC was present; ▾, 10 μg/ml BSA was used as a negative control. D–K, in vitro crystallization experiments in the presence of truncations and peptides. 10 μg/ml Pep-C was added with (F) or without (D) the silk film. 10 μg/ml ΔC was added with (J) or without (H) the silk film. E, G, I, and K, enlargement of boxes in D, F, H, and J, respectively. Scale bars, 50 μm in D, F, H, and J and 5 μm in E, G, I, and K.