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. 2012 Mar 19;287(19):16007–16016. doi: 10.1074/jbc.M111.337485

FIGURE 4.

FIGURE 4.

FCA mediates temperature regulation of miR172 processing. Ten-day-old whole plants grown on MS-agar plates at either 23 or 16 °C were used for extraction of total RNA. miR172 levels were examined by Northern blot analysis. Error bars indicate S.E. A, effects of ambient temperature on MIR172 gene expression. Transcript levels of the MIR172 genes were analyzed by qRT-PCR (t test; *, p < 0.05). B, relative levels of pri-miR172 and pre-miR172 transcripts and mature miR172. miR167 was included for comparison. The asterisk indicates the pri-miR172-to-pre-miR172 processing step (right panel). The numbers indicate molecular sizes (in bases) of size marker (SM) RNAs. C, MIR172b gene constructs used. Black boxes are introns, and white boxes are exons. b, bases. D, miR172 levels in 35S:pri-miR172b transgenic plants. E, miR172 levels in 35S:pre-miR172b transgenic plants. F, miR172 levels in flowering time mutants overexpressing pri-miR172b construct driven by the cauliflower mosaic virus 35S promoter. Rel., relative.