Skip to main content
. 2012 Mar 19;287(19):16007–16016. doi: 10.1074/jbc.M111.337485

FIGURE 6.

FIGURE 6.

FCA promotes processing of pri-miR388 and pri-miR399 transcripts. The assays were carried out as described in Fig. 5. A, levels of miR156, miR172, miR398, and miR399. Two-week-old plants grown at 23 °C on MS-agar plates were harvested at ZT16. miRNA levels were examined by Northern blot analysis (t test; *, p < 0.01). Error bars indicate S.E. B, prio-miR398c and prio-miR399a RNAs assayed. b, bases. C, in vitro pulldown assays on FCA binding to prio-miR398c and prio-miR399a RNAs. The in vitro transcribed and biotinylated prio-miR398c and prio-miR399a RNAs were used. kDa, kilodaltons. D and E, EMSAs on FCA binding to prio-miR398c (D) and prio-miR399a (E) RNAs. F and G, in vitro binding of FCA to prio-miR398c (F) and prio-miR399a (G) RNAs. H, RIP assays on in vivo binding of FCA to pri-miR398 and pri-miR399 transcripts. Rel., relative; Ab, antibody.