FIGURE 7.

FCA regulates miR172 processing in thermosensory flowering. In A–C, mRNA levels were determined by qRT-PCR (t test; *, p < 0.05). Error bars indicate S.E. A, effects of ambient temperature on the accumulation of FCAγ mRNA. qRT-PCRs were carried out using the plant materials described in supplemental Fig. S1. B, kinetic measurements of FCAγ mRNA levels after transfer from 23 to 16 °C. Relative levels of FCAγ mRNAs were determine using the cDNA samples of Col-0 plants described in Fig. 2C. C, effects of ambient temperature on FCA protein levels. The 35S:FCA transgenic plants were grown on MS-agar plates at either 23 or 16 °C for 10 days before harvesting whole plants. FCA protein was detected immunologically using an anti-MYC antibody (top panel). The numbers refer to the relative protein levels (middle panel). Relative levels of FCAγ mRNAs were determined by qRT-PCR (bottom panel). Rub, ribulose-bisphosphate carboxylase/oxygenase. D, schematic model of FCA function in thermosensory regulation of miR172 processing. Rel., relative.