FIGURE 2.

Interaction studies of Hcp with TssL and TssM. A, the cell lysate of E. coli co-expressing Hcp with full-length TssM tagged with HA (TssM-HA) was subjected to co-immunoprecipitation using anti-HA beads. B, the cell lysate of E. coli expressing Hcp alone or in the presence of full-length TssL tagged with His (TssL-His), the C-terminal periplasmic domain of TssL-His (TssL(Peri)-His), or the N-terminal cytoplasmic domain of TssL (TssL(Cyto)-HA) were purified by either Ni²⁺-NTA affinity chromatography or co-immunoprecipitation using anti-HA beads. The load (L), flow-through (F) and washed (W) and bound (B) fractions were analyzed by immunoblotting with antisera against C-TssM, N-TssL, Hcp, or His₆ (for TssL(Peri)-His), respectively. The sizes of the molecular mass standards (kDa) are indicated.