FIGURE 1.

Phenotypic and molecular characterization of OE-AHL22 and ahl22 mutants. A, phenotypic comparison. Plants were grown in soil for 4 weeks under LDs before taking photographs. The AHL22 gene was overexpressed driven by the CaMV 35S-promoter in Col-0 plants, resulting in 35S:AHL22 transgenic plants. B, mapping of the T-DNA insertion events. The AHL22 gene does not have introns. C, FT transcript levels. Transcript levels were determined by qRT-PCR. Biological triplicates were averaged and statistically treated using a Student's t test (*, p < 0.01). Bars indicate S.E. of the mean. D and E, expression domains of AHL22 and FT genes. Whole-mount staining of 8-day-old seedlings (D) and staining of the first rosette leaves of 12-day-old seedlings (E) were displayed.