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Modifications of FT chromatin by AHL22. Relative levels of H3 modifications in FT chromatin were examined by ChIP assays using an anti-H3Ac (A), -H3K9me2 (B), or -H3K27me3 (C) antibody. PCR primer pairs specific to M1, M2, and M3 sequences, as shown in Fig. 2A, were used. Plants grown on MS-agar plants for 12 days under LDs were used for chromatin preparations. Three measurements were averaged and statistically treated (t test, *, p < 0.01). Bars indicate mean ± S.E.
