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α-Actinin and CaMKII exist in complex with NMDARs in mouse brain. A mouse forebrain Triton-soluble fraction (TSF) was immunoprecipitated (IP) using CaMKII, α-actinin, or control IgG. The Triton-insoluble fraction (TIF), TSF and immune complexes were immunoblotted for GluN2B, GluN1, α-actinin, and CaMKII as indicated.
