α-Actinin modulates phosphorylation of GluA1 and GluN2B by CaMKII.
A, GluA1 was immunoprecipitated from lysates of HEK293 cells expressing GluA1 with or without TT305/6AA-CaMKIIα and HA-actinin-2 (WT) and immunoblotted for total GluA1 and phospho-Ser831 (P-S831). B, lysates of HEK293 cells expressing GluN2B with or without TT305/6AA-CaMKIIα and HA-actinin-2 were directly immunoblotted for total GluN2B, phospho-Ser1303 (P-S1303), CaMKII and HA as indicated. Graphs plot phospho-protein levels as a ratio to corresponding total protein after normalization to total GluA1 alone (A) or total GluN2B+AACK (B) (mean ± S.E., n = 4. *, p < 0.01 by 1-way ANOVA with Bonferoni post-test).