Skip to main content
. 2001 May 22;98(11):6295–6300. doi: 10.1073/pnas.111152498

Figure 1.

Figure 1

Neonatal microglia can be skewed toward a DC-like or macrophage-like phenotype by GM-CSF or M-CSF. (A) Neonatal microglia express c-kit, but not flt3, as analyzed by flow cytometry (c-kit, clone 2B8; flt3, A2F10.1). (B) Phenotypic markers are differentially expressed on neonatal microglia after administration of myeloid growth factors. Neonatal microglia were cultured with or without 5 ng/ml GM-CSF or 1 ng/ml M-CSF from the beginning of the mixed glial culture. (Upper) Phase-contrast confocal microscopy of microglia skewed as described above (magnification: microglia, ×69; GMCSF and MSCF, ×100). (Lower) Cell-surface expression of CD11b (clone M1/70), CD11c (clone HL3), mannose receptor (clone Dec205), B7–1 (clone 16–10A1), and B7–2 (clone GL1) was analyzed by flow cytometry.