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. 2012 Jan 24;63(7):2421–2435. doi: 10.1093/jxb/err397

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© 2012 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 4. — Influence of virus-induced gene silencing (VIGS)-inducing treatment for six genes on growth and development of N. benthamiana plants. Genes subjected to VIGS analyses encoded (A–C) a proteasome 20S beta 1.1 subunit (NbPb, 19), (D) a luminal-binding protein (NbBiP, 58), (E, F) a SIPK type MAP kinase (Nbf4, b12), (G, H) an asparagine synthetase (NbAsn, b48), (I) an ASR (Abscissic acid, Stress, Ripening) protein (NbASR, b67), and (J) a chloroplast thiazole biosynthetic protein (NbTHI, b73). Plants were infiltrated with cell suspensions of Agrobacterium transformed with TRV VIGS vector carrying a fragment of the target gene or just empty vector (EV; K, L). The photographs were taken 12 days (A, B), 14 days (D, K), 19 days (C), 21 days (G, J), 30 days (I, L), and 42 days (E, F, H) after agroinfiltration. Spot numbers at bottom right are as given in Supplementary Table S3.