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. 2012 Jan 16;63(7):2465–2478. doi: 10.1093/jxb/err416

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© 2012 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 2. — Determination of flavonols from Col-0, ugt78d1, ugt78d2, and ugt78d1 ugt78d2 by HPLC analyses. (A) Representative HPLC diagrams of the flavonol glycoside profiles from leaves of 3-week-old plants. IS, internal standard (naringenin); SM, sinapoyl malate; SG, sinapoyl glucose; f1, f2, and f3 are as described in the legend of Fig. 1. Arrows indicate missing or repressed glycosides. (B) Representative HPLC diagrams of flavonol aglycone profiles from leaf extracts after acid hydrolysis. Q, quercetin; K, kaempferol; I, isorhamnetin. (C) Total flavonol aglycone quantification after acid hydrolysis of the methanolic extracts from leaves and roots. Means ±SD determined from three independent experiments are shown.