Table 1.
Mass spectra of products of the methyl ester trimethylsilylether derivatives from incubations of [14C]GAs with cell lysates from Escherichia coli expressing recombinant pumpkin GA20ox4, GA3ox4, GA2ox2, and GA2ox3
| Enzyme | Substrate | Compound formed | Mass spectrum m/z (relative intensity) |
| GA20ox4 | [1-,7-,12-,18-14C]GA12 | [1-,7-,12-,18-14C]GA15 | M+ 352(5), 344(3), 320(11), 312(6), 306(8), 298(4), 290(34), 284(20), 245(100), 239(52), 201(47), 195(22) |
| [1-,7-,12-,18-14C]GA24 | M+ 382(0), 350(11), 342(6), 322(39), 314(30), 293(28), 285(16), 232(86), 231(100), 226(49), 225(52) | ||
| [1-,7-,12-,18–14C]GA9 | M+ 338(6), 330(3), 306(75), 298(45), 276(100), 270(54), 251(60), 243(42), 233(66), 232(78), 227(38), 226(48), 189(48), 183(36) | ||
| [17-14C]GA24 | [17-14C]GA9 | M+ 332(4), 300(87), 288(11), 272(100), 229(74), 228(88), 213(32), 185(47) | |
| [17-14C]GA25 | M+ 406(0), 374(12), 346(1), 314(66), 286(100), 271(10), 255(14), 227(77) | ||
| GA3ox4 | [17-14C]GA9 | [17-14C]GA4 | M+ 420(11), 388(18), 360(11), 330(27), 291(63), 286(100), 263(35), 235(62) |
| GA2ox2 | [17-14C]GA4 | [17-14C]GA34 | M+ 508(100), 418(8), 389(12), 374(13), 359(14), 358(17), 329(15), 315(25), 290(31), 263(13), 231(59), 225(64), 203(71) |
| [17-14C]GA34 catabolite (as ketone) | M+ 448(28), 373(62), 341(15), 329(100), 313(77), 299(38), 260(51), 239(69), 201(74) | ||
| [17-14C]GA9 | [17-14C]GA51 | M+ 420(1), 388(15), 330(15), 298(19), 286(67), 270(75), 243(29), 229(45), 227(100), 226(55), 225(28), 143(44) | |
| [17-14C]GA51 catabolite (as enol) | M+ 432(100), 417(3), 373(15), 357(17),313(79), 283(14), 269(26) | ||
| GA2ox3 | [17-14C]GA4 | [17-14C]GA34 | M+ 508(100), 418(9), 389(11), 374(11), 359(13), 358(12), 329(10), 315(27), 290(33), 263(27), 231(72), 225(79), 203(80) |
| [17-14C]GA9 | [17-14C]GA51 | M+ 420(1), 388(14), 330(14), 298(18), 286(67), 270(75), 243(28), 229(44), 227(100), 226(56), 225(30), 143(44) | |
| [17-14C]GA51 catabolite (as enol) | M+ 432(100), 417(2), 373(10), 357(12), 313(82), 283(14), 269(20) |