Fig. 2.

Identification of WRKY group III transcription factor interactions with yeast two-hybrid analysis. A split-ubiquitin system was used to screen interactions. Yeast strain NMY51 was co-transformed with various bait and prey constructs as indicated and plated on SD medium without Leu and Trp (line 1: all transformed yeast grown with red/white colonies depending on protein interactions) and without Leu, Trp, His, and Ade (line 2: transformed yeast grown depending on protein interactions). Each transformed yeast line was used to perform X-gal assays on the pellet (line 3). The largeT gene was used as bait control. Vectors carrying NubI or NubG were used as a prey control for negative and positive interactions, respectively.