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. 2012 Jan 20;63(7):2667–2679. doi: 10.1093/jxb/err450

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© 2012 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 4. — Characterization of WRKY transgenic lines. (A) Schematic representation of WRKY54 and WRKY70 gene structure indicating the location of T-DNA insertions. Exons are shown as dark boxes. The grey part indicates the region encoding the WRKY domain. (B) RT-qPCR analysis of WRKY54 and WRKY70 transcript levels in wrky54/wrky70 single and double mutants sprayed with 5 mM SA, compared with wild-type plants. Measurements were done 5 h after treatment. (C) RNA gel blot analysis of the WRKY30 transcript level in two independent miRNA-WRKY30 lines sprayed with 5 mM SA compared with wild-type plants. Measurments were done 5 h after treatment. EtBr (ethidium bromide) staining of the gel was used as loading control.