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. 2012 Jan 30;63(7):2809–2823. doi: 10.1093/jxb/err466

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© 2012 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 2. — Expression patterns of SmDXS genes and the phylogenetic relationship of their deduced proteins with various other plant species. (A) Phylogenetic relationship of plant DXSs. The rooted Neighbor–Joining tree was constructed using the MEGA program (version 4.0) with default parameters. CrDXS (Chlamydomonas reinhardtii, CAA07554) was used as an outgroup. Transit peptides of DXSs were trimmed for the analysis of sequence data. DXSs included are Arabidopsis thaliana AtDXS1 (At4g15560), AtDXS2 (At3g21500), AtDXS3 (At5g11380), Oryza sativa OsDXS1 (NP_001055524), OsDXS2 (NP_001059086), OsDXS3 (BAA83576), Populus trichocarpa PtDXS1 (XP_002312717), PtDXS2A (XP_002303416), PtDXS2B (XP_002331678), PtDXS3 (XP_002308644), Picea abies PaDXS1 (ABS50518), PaDXS2A (ABS50519), PaDXS2B (ABS50520), and five S. miltiorrhiza SmDXSs (highlighted). (B) Fold changes of SmDXS genes in flowers (Fl), leaves (Le), stems (St), root cortices (Rc), and root steles (Rs) of S. miltiorrhiza plants grown in soil. The expression level of SmDXS1 in root steles was arbitrarily set to 1. (C) Fold changes of SmDXS genes in leaves (L) and roots (R) of S. miltiorrhiza plantlets treated with MeJA for 0 h and 24 h. The level of SmDXS1 in roots of plantlets without treatment was arbitrarily set to 1.