Histological liver necrosis after acute ethanol ingestion after at 2 h, 24 h and 72 h after H/R. Rats were gavaged with saline (Ctrl) or ethanol (5 g·kg−1, 30% EtOH), 12 h before H/R. Sham-operated animals underwent the same surgical procedures but H/R was not carried out. Representative haematoxylin- and eosin- stained liver lobes from saline-treated (Ctrl) (A: after sham operation, B, C and D, 2, 24 and 72 h after H/R, respectively) or ethanol-treated (E: after sham operation, F, G and H, 2, 24 and 72 h after H/R) rats are shown. Bar is 200 µm.