Figure 3.

Cpd1 and Cpd2 activate transcription via GC-responsive regions of the human DUSP1 gene. HeLa cells were transiently transfected with pGL3p or derivatives that contain GRR-1.3 or GRR-4.6 from the human DUSP1 gene. A plasmid expressing Renilla luciferase was co-transfected as a control for transfection efficiency. Cells were treated with vehicle (0.1% DMSO) or 10⁻⁷ M GR ligands as indicated for 6 h, then lysates were prepared, firefly and Renilla luciferase activities were measured and fold responses to ligand were calculated. The graph represents mean ± SEM from three (pGL3p), six (pGL3p-GRR-1.3) or seven (pGL3p-GRR-4.6) independent experiments. ***P < 0.005; **P < 0.01; *P < 0.05 relative to vehicle-treated cells.