Table 1.
BACE1 antibodies screened by Western blot.
| Antibody | Provider (Cat. #) | Epitope | Tissue tested | Western blot results |
|---|---|---|---|---|
| Goat anti-BACE1 ectodomain | R&D Systems (AF931) | 22–460 | Human & mouse brains | Low signals, bands at 55 and 70 kDa |
| Mouse anti-BACE1 C-term | Millipore (MAB5308) | C-term | Human & mouse brains | Low signals, bands at 55 and 70 kDa |
| Mouse anti-BACE1 ectodomain | R&D Systems (MAB 9311) | 22–460 | Human & mouse brains | No signal |
| Mouse anti-BACE1 ectodomain | R&D Systems (MAB931) | 22–460 | Human & mouse brains | Strong signal for bands at 55 and 70 kDa |
| Rabbit anti-BACE1 C-term | Calbiochem (195102) | 487–501 | Mouse brain | Several unspecific extra-bands |
| Rabbit anti-BACE1 C-term | Sigma (B0806) | 485–501 | Human brain | Low signals, bands at 55 and 70 kDa |
| Rabbit anti-BACE1 C-term | Thermo Fisher (PA1-757) | 485–501 | Human brain | Very low signals |
| Rabbit anti-BACE1 N-term | Calbiochem (195101) | 46–65 | Mouse brain | Several unspecific extra-bands |
| Rabbit anti-BACE1 N-term | IBL (28051) | 42–57 | Human & mouse brains | Very low signals |
| Rabbit anti-BACE1 N-term | Sigma (B0681) | 46–62 | Human & mouse brains | Strong signal for bands at 55 and 70 kDa |