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. Author manuscript; available in PMC: 2013 May 18.
Published in final edited form as: J Mol Biol. 2012 Mar 3;418(5):367–378. doi: 10.1016/j.jmb.2012.02.024

Figure 3.

Figure 3

Constitutive levels of induction of expression of the FUS1 lacZ reporter. a) Effect of DTT on constitutive activity of various receptor alleles. Unfilled bars refer to β-galactosidase activity measured following treatment of intact cells treated with 0.5 mM DTT for 2 hours. Filled bars indicate activities of receptors expressed on cells that were not treated with DTT. The β-galactosidase activities of all mutants were normalized to the untreated normal Cys-less basal activity. b) Requirement for the presence of two cysteines to maintain full constitutive induction of FUS1-lacZ reporter. The graph shows the constitutive β-galactosidase activity induced by alleles containing two cysteine residues in comparison to alleles containing only one cysteine, Cys-less alleles, and the previously-characterized constitutive allele containing the P258L substitution.