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. Author manuscript; available in PMC: 2013 May 18.
Published in final edited form as: J Mol Biol. 2012 Mar 3;418(5):367–378. doi: 10.1016/j.jmb.2012.02.024

Figure 4.

Figure 4

Detection of disulfide bonds by immunoblotting of C-terminally c-myc tagged CNBr-cleaved alleles of Ste2p. a) Immunoblot of CNBr cleaved Ste2p alleles containing different cysteine pairs involving TM5 and TM6 that result in constitutive activity Where indicated, samples were incubated with 200 mM DTT at 37° C for 15 minutes prior to SDS polyacrylamide gel electrophoresis. b) Immunoblot of receptors containing cysteine pairs involving TM5 and TM6 that form disulfides but do not result in constitutive activation. c) Immunoblot of receptors containing cysteine pairs in TM5 and TM6 that do not form disulfides.