Figure 2.

Anti-Qa-1a antiserum, antimurine CD8 mAb, and antimurine TCR mAb block the specific recognition of TH1 Vβ8⁺CD4⁺ T cell clone 1AE10 by the CD8⁺ T cell hybridoma 21–5A9. In these experiments, varying amount of either (a) murine anti-Qa-1a or control anti-Qa-1b sera; (b) rat anti-mouse CD8 mAb (53–6.72) or control rat Ig; and (c) hamster anti-mouse TCR mAb (H57) or control hamster Ig were added to the coculture of 1AE10 and 21–5A9. Reactivity of 21–5A9 was assayed as described in Fig. 1. Inhibition of 21–5A9 reactivity was assessed as a decrease in the percent response compared with the response of the 21–5A9 hybridoma without antibody or control Ig.