Figure 5.

CD8⁺ T cells control the TH phenotype of 1–9Nac MBP-reactive CD4⁺ T cells in EAE mice. The TH phenotype of 1–9Nac MBP-reactive CD4⁺ T cells in EAE recovered mice (EAE) and CD8⁺ T cell-depleted EAE recovered mice (CD8-/EAE) were assayed and compared. The CD4⁺ T cells from the EAE mice and CD8-/EAE mice were isolated and assayed for TH phenotype by intracellular staining by FACS analysis for the expression of intracellular IFN-γ and IL-5 as described. Results are representative of four separate experiments.