Table 1.

Cytokines or other soluble TH products do not influence the TH specificity of the 21-5A9 CD8⁺ T cell hybridoma

	Stimulator cells
	1AE10 Vβ8 TH1	3AF9 Vβ6 TH1	3AD2 Vβ8 TH2
	Response of 21-5A9 (A570–A650)
Experiment A*
IL-2 starved	0.157	0.073	0.064
IL-2 10 units/ml	0.602	0.099	0.067
IL-2 + IFN-γ 200 units/ml	0.650	0.108	0.075
IL-2 + IFN-γ 20 units/ml	0.590	0.107	0.074
IL-2 + IL-4 200 units/ml	0.602	0.091	0.079
IL-2 + IL-4 20 units/ml	0.596	0.106	0.081
IL-2 + 48 h TH1 sups	0.740	0.104	0.033
IL-2 + 96 h TH1 sups	0.523	0.086	0.058
Fresh cells (control)	0.460	0.049	0.039
Experiment B†
IL-2 alone	0.314	0.028	0.026
IL-2 + 96 h TH1 sups	0.373	0.071	0.076
IL-2 + 96 h TH2 sups	0.352	0.054	0.065
Fresh cells (control)	0.357	0.044	0.033
Cell mixing experiments†
1AE10 + 3AF9		0.297
1AE10 + 3AD2		0.374
3AD2 + 3AF9		0.062

^*Representative of three separate experiments. 

^† Representative of two separate experiments.