Table 2. Oligonuclotide probes used in Northern hybridizations.
sRNA | Nucleotide sequence | Target sequencea |
---|---|---|
speF |
5′-CTTCAGTAACCCCGGCTGTTGGAGA-3′ |
3,105,282–3,105,258 |
Smr45C |
5′-CCGCACCGTCGTTGCTTCAAGATGT-3′ |
3,105,328–3,105,304 |
Smr14C1 |
5′-AACCGACCGAATGCCGGGCGCCGTG-3′ |
206,954–206,930 |
Smr14C2 |
5′-TGCTTGATCTGATTGGCAACCGGGA-3′ |
1,667,552–1,667,528 |
Smr14C3 |
5′-ACCGGCGGGCGTCATAAAGGCGATT-3′ |
1,667,818–1,667,794 |
Smr14A1 |
5′-AACCGATCGGCGTCTTGCGCCGTGG-3′ |
1,220,715–1,220,739 |
Smr14A2 |
5′-GAGGAAAGGTCGCTCGCATATCGAA-3′ |
1,328,303–1,328,279 |
Smr14B |
5′-GTGCGCCGGGCTTTCGATCCTGACC-3′ |
1,605,895–1,605,919 |
Smr15C1 |
5′-GAGGAGAAAGCCGCTAGATGCACCA-3′ |
1,698,728–1698,704 |
Smr15C2 |
5′-ACTGGGAGGAGAAGCCACCAAAGAT-3′ |
1,698,928–1698,904 |
Smr15A | 5′-GGAGGAAAACTGCCATGCGCATCAA-3′ | 552,875–552,899 |
a Coordinates of the sequence stretches complementary to each probe in the S. meliloti 1021 genome according to iant.toulouse.inra.fr/bacteria/annotation/cgi/rhime.cgi.