Vector, pGRA7, pROP1, and pGRA7-ROP1 were localized to the cytoplasm of transfected cells. ARPE-19 cells were transfected with vector (A), pGRA7 (B), pROP1 (C), or pGRA7-ROP1 (D). Cells were fixed 48 h after transfection, anti-flag antibody (red) was used to evaluate the expression of protein levels, and nuclear DNA was stained with DAPI (shown in blue).