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. 2012 May;19(5):666–674. doi: 10.1128/CVI.05385-11

Fig 5.

Fig 5

Expression and purification of recombinant proteins. Proteins were submitted to SDS-PAGE and revealed by Coomassie blue staining. (A) Lane 1, pGEX-4T-1-transfomed E. coli; lane 2, IPTG-induced, pGEX-4T-1-transfomed E. coli soluble fraction; lane 3, purifed GST protein (26 kDa). (B) Lane 1, pGEX-GRA7 transfomed E. coli; lane 2, IPTG-induced, pGEX-GRA7-transfomed E. coli soluble fraction; lane 3, purifed GRA7 protein (53 kDa). (C) Lane 1, pGEX-ROP1-transfomed E. coli; lane 2, IPTG-induced, pGEX-ROP1-transfomed E. coli soluble fraction; lane 3, purifed ROP1 protein (69 kDa). (D) Western blot analysis of purified recombinant proteins with anti-GST primary Ab. Lane 1, GST; lane 2, GRA7; lane 3, ROP1. M, protein molecular size marker.