Table 2.

Cytokine concentrations of culture supernatants of the splenocytes from mice immunized with empty vector, pIL12, pGRA7, and/or pROP1 by ELISA

Group	Cytokine concna (pg/ml)
	GST antigen stimulated				GRA7 and ROP1 antigen stimulated
	IL-4	IL-10	IFN-γ	TNF-α	IL-4	IL-10	IFN-γ	TNF-α
Vector	34 ± 4	36 ± 14	39 ± 5	20 ± 8	39 ± 3	37 ± 22	42 ± 4	36 ± 2
pIL12	35 ± 7	36 ± 8	41 ± 4	27 ± 4	46 ± 8	41 ± 10	43 ± 6	35 ± 4
Vector+pIL12	33 ± 15	34 ± 13	40 ± 6	26 ± 5	50 ± 5	42 ± 7	44 ± 5	38 ± 6
pGRA7	38 ± 12	68 ± 16	89 ± 14*	54 ± 11*	42 ± 7	191 ± 29*	317 ± 51*	323 ± 59*
pROP1	35 ± 2	77 ± 13	83 ± 8*	65 ± 7*	36 ± 2	184 ± 33*	327 ± 37*	286 ± 31*
pGRA7-ROP1	36 ± 3	94 ± 11*	124 ± 26*	88 ± 12*	36 ± 3	250 ± 70*	420 ± 34*	471 ± 73*
pGRA7+pIL12	34 ± 7	86 ± 11*	107 ± 16*	65 ± 10*	34 ± 5	214 ± 25*	345 ± 29*	343 ± 68*
pROP1+pIL12	36 ± 7	77 ± 23	118 ± 13*	71 ± 12*	38 ± 3	249 ± 33*	440 ± 20*	451 ± 47*
pGRA7-ROP1 + pIL12	35 ± 3	101 ± 21*	140 ± 17*	109 ± 18*	36 ± 4	410 ± 42*	494 ± 42*	641 ± 52*

^aValues for IL-4, IL-10, IFN-γ, and TNF-α are for 24, 72, 96, and 48 h, respectively. Data represent the means ± SD from 3 mice per group. Splenocytes from mice were harvested 4 weeks after the last immunization. Single-cell suspensions of splenocytes were stimulated by incubation with purified GST antigen alone or in combination with recombinant GRA7 and ROP1 antigens (5 μg/ml). Three independent experiments were performed, and data from one representative experiment are shown. *,P < 0.05 compared to mice immunized with empty vector.