Table 3.
Cytokine production by splenocytes of immunized Kunming mice after stimulation by STAga
| Group | Cytokine production (pg/ml)b |
||||
|---|---|---|---|---|---|
| IFN-γ | IL-2 | IL-12 | IL-4 | IL-10 | |
| pIRESneo/MIC6/PLP1+pVAX/IL-18 | 886.5 ± 9.5 A | 413.5 ± 9.5 A | 329.8 ± 18.8 A | 150.8 ± 9.5 A | 103.5 ± 4.0 B |
| pIRESneo/MIC6/PLP1 | 763.5 ± 12.3 B | 304.0 ± 9.4 B | 239.3 ± 16.7 B | 164.0 ± 15.1 A | 151.8 ± 11.4 A |
| pVAX/PLP1 | 471.5 ± 28.9 D | 206.3 ± 28.2 D | 130.3 ± 17.7 D | 118.5 ± 6.4 B | 67.3 ± 2.9 C |
| pVAX/MIC6 | 475.8 ± 21.2 D | 208.0 ± 7.2 D | 130.5 ± 7.51 D | 115.5 ± 7.9 B | 68.3 ± 1.9 C |
| pVAX/IL-18 | 616.8 ± 50.7 C | 259.8 ± 6.0 C | 190.0 ± 6.8 C | 57.5 ± 3.1 C | 33.8 ± 2.3 D |
| pIRESneo | 57.0 ± 2.4 E | 59.0 ± 0.9 E | 57.5 ± 2.3 E | 50.5 ± 1.2 C | 40.5 ± 1.2 D |
| pVAX I | 56.5 ± 4.2 E | 56.3 ± 3.9 E | 54.5 ± 3.4 E | 49.0 ± 1.6 C | 40.8 ± 1.0 D |
| PBS | 55.8 ± 1.8 E | 55.5 ± 12.9 E | 55.5 ± 3.6 E | 50.0 ± 2.7 C | 39.0 ± 0.8 D |
a
Splenocytes from nine mice were harvested 2 weeks after the last immunization.
b
Values for IFN-γ are for 96 h poststimulation; values for IL-2 and IL-4 are for 24 h; values for IL-10 and IL-12 are for 72 h. Data followed by different capital letters in the same column are significantly different from each other (P < 0.05).